Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), NovaFluor Yellow 660, eBioscience

Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), NovaFluor Yellow 660, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.4 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661 / 737 / 805
• Brilliant Violet 480
• eFluor 450 / 506
• FITC
• Functional Grade
• NovaFluor Blue 610-70S / Blue 660-120S / Red 710 / Red 725 / Yellow 730
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation available

Product Specific Information

Description:
The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), a low-affinity receptor for IgG (50–80 kDa). CD16 includes two genes: CD16A (FcγRIIIA) in macrophages and NK cells, and CD16B (FcγRIIIB) in neutrophils. CD16 binds IgG immune complexes, preferentially IgG1 and IgG3, and mediates ADCC, phagocytosis, degranulation, and proliferation.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells.
Use at 5 µL (0.4 µg) per test (100 µL final volume). Cell count: 10⁵–10⁸ cells/test.

Compatibility Note:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01):
Required for optimal NovaFluor staining. Add 5 µL per sample (regardless of number of NovaFluors).
For single-color controls: use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor Conjugate Technology:
Based on Phiton™ technology using nucleic acid dye structures engineered for optimized fluorescent signatures and minimized spillover/spread.

Excitation / Emission / Laser:

• Excitation: 552 nm
• Emission: 663 nm
• Laser: 561 nm (Yellow)

Target Information

CD16 encodes an Fc receptor for IgG involved in immune complex clearance, ADCC, and viral infection modulation. FCGR3A (CD16A) is expressed on NK cells (transmembrane glycoprotein), while FCGR3B (CD16B) is expressed on neutrophils (PI-linked).
Mutations are associated with immunodeficiency 20, recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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