
Thermo Fisher Scientific CD105 (Endoglin) Monoclonal Antibody (SN6), eFluor 450, eBioscience
인간 CD105(Endoglin)을 인식하는 SN6 클론 단일클론 항체로, eFluor 450 형광체로 표지되어 있습니다. 유세포 분석에 최적화되어 있으며, 405 nm Violet 레이저로 여기됩니다. 혈관 내피세포 및 종양 혈관 연구에 적합합니다.
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Thermo Fisher Scientific CD105 (Endoglin) Monoclonal Antibody (SN6), eFluor 450, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 61 publications |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Fruit fly, Human, Mouse |
| Host/Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SN6 |
| Conjugate | eFluor™ 450 |
| Excitation/Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11218703 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Brilliant UV 661
- Functional Grade
- PE
- PE-Cyanine7
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Custom conjugation available
Product Specific Information
The SN6 monoclonal antibody reacts with human CD105 (Endoglin), a ~90 kDa disulfide-linked homodimer expressed by vascular endothelial cells, bone marrow cells, and activated macrophages. CD105 is involved in adhesion and embryonic angiogenesis.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and validated using U937 cell line at 5 µL (0.25 µg) per test
- Test Definition: Amount of antibody to stain cells in 100 µL final volume (10⁵–10⁸ cells/test)
Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emitting at 445 nm. Ensure instrument compatibility with Violet laser detection.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD105 (Endoglin) is a Type I transmembrane protein highly expressed on human vascular endothelial cells. It forms an O- and N-glycosylated homodimer and is upregulated in tumor vasculature and proliferating cells, serving as a proliferation-associated endothelial marker. CD105 binds TGF-β1 and TGF-β3 with high affinity, but not TGF-β2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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