Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (429), eFluor 450, eBioscience
마우스 CD106(VCAM-1)을 인식하는 단클론 항체로, eFluor 450 형광체로 결합되어 Flow Cytometry에 최적화됨. 405/445 nm 파장에서 검출 가능하며, 염증 반응 및 세포 부착 연구에 활용 가능. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (Clone 429), eFluor 450
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 8 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 429 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1633378 |
Available Formats
- Unconjugated
- APC
- Biotin
- eFluor 660
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- Custom conjugation available upon request
Product Specific Information
The 429 monoclonal antibody reacts with mouse CD106 (VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to integrin α4β1 (VLA-4, CD49d/CD29) and integrin α4β7 (LPAM-1), interactions that are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role in the inflammatory response.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Mouse bone marrow cells, ≤ 0.25 µg/test (100 µL final volume)
- Recommended: Empirical titration for optimal performance
- Filtration: 0.2 µm post-manufacturing filtered
eFluor® 450 is an alternative to Pacific Blue®, excited by the violet laser (405 nm) and emits at 445 nm. Ensure instrument compatibility.
Target Information
VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. It regulates leukocyte migration, adhesion, and signal transduction, and may be involved in inflammatory diseases.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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