Thermo Fisher Scientific CD103 (Integrin alpha E) Monoclonal Antibody (2E7), eFluor 450, eBioscience
마우스 CD103(Integrin alpha E)을 인식하는 2E7 클론 단일클론 항체로, eFluor 450 형광체로 결합되어 흐름세포분석에 적합. Violet laser(405 nm)에서 여기되어 445 nm에서 방출. 고순도 친화 크로마토그래피 정제, 4°C 암소 보관, 연구용 전용.
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Thermo Fisher Scientific CD103 (Integrin alpha E) Monoclonal Antibody (2E7), eFluor 450, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | View 20 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), eFluor™ 450 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2E7 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574032 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 563 / 615 / 737
- Brilliant Violet 421 / 480 / 711
- FITC
- Functional Grade
- NovaFluor Blue 510
- PE / PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600
- Custom conjugation available
Product Specific Information
The 2E7 monoclonal antibody reacts with mouse CD103, the alphaE integrin. CD103 non-covalently associates with integrin beta7 and is expressed on intraepithelial lymphocytes, a subset of peripheral lymphocytes, and dendritic epidermal T cells (DEC).
E-cadherin binds to CD103 and mediates lymphocyte homing to intestinal epithelium.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse lymph node cells. Recommended ≤1 µg/test (final volume 100 µL). Cell number may range from 10⁵–10⁸ cells/test. Optimize titration for best results.
Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®. Excitation: 405 nm; Emission: 445 nm; Laser: Violet. Ensure instrument compatibility.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD103 (ITGAE, Integrin alpha E) is expressed on mucosa-associated T lymphocytes and activated cells. It comprises a 150 kDa alpha chain and a 120 kDa beta chain.
CD103 is expressed by 0.5–2% of resting lymphocytes in peripheral blood and lymphoid organs. It is part of the integrin family, composed of heterodimeric alpha and beta chains.
ITGAE encodes an I-domain-containing alpha integrin that undergoes extracellular cleavage, forming disulfide-linked heavy and light chains.
In combination with beta7 integrin, CD103 forms the E-cadherin binding integrin known as mucosal lymphocyte-1 antigen.
CD103 is preferentially expressed in human intestinal intraepithelial lymphocytes and may serve roles in adhesion and activation.
Associated diseases: hairy cell leukemia, splenic marginal zone lymphoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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