Thermo Fisher Scientific MINT3 Polyclonal Antibody
MINT3 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, Western blot, IHC, ICC/IF, IP 등에 사용 가능. 인간 시료 반응성, E. coli 유래 APBA3 항원으로 제작. 연구용으로만 사용.
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Thermo Fisher Scientific MINT3 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Immunoprecipitation (IP) | 1–5 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived Human APBA3 fragment |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography, Protein A |
| Storage Buffer | PBS, pH 7 |
| Contains | 0.03% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2902397 |
Target Information
The munc-18 interacting protein (Mint) family comprises evolutionarily conserved adaptor proteins involved in membrane transport and organization. Mammals express three isoforms: Mint1, Mint2, and Mint3. While the N-terminal region shows little sequence conservation, the C-terminal region is highly conserved and contains phosphotyrosine-binding (PTB) and PSD-95/DLG-A/ZO-1 (PDZ) domains, which serve as protein interaction modules.
Mint1 and Mint2 are expressed mainly in the brain and interact with Munc18, a key component of synaptic vesicle fusion. Mint3, by contrast, is ubiquitously expressed, with lowest levels in brain and testis, and does not interact with Munc18. Mint3 interacts with the Alzheimer’s disease-related amyloid precursor protein (APP) through its PTB and PDZ domains, potentially linking APP to transport machinery components and regulating its trafficking, endocytosis, and metabolism. Abnormal APP metabolism is associated with early-onset Alzheimer’s disease.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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