Thermo Fisher Scientific WNT3A Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific WNT3A Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Wnt-3a. Anti-Human Wnt-3a-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hWNT-3a by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human WNT-3a (500-P251) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hWNT-3a.

Western Blot:
To detect hWNT-3a by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hWNT-3a is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package Information:
500-P251BT-1MG will be provided as 2 × 500 µg.

Target Information

Wnt3a is a member of the WNT gene family, which encodes secreted signaling proteins involved in oncogenesis and developmental processes such as cell fate regulation and patterning during embryogenesis. Expression studies in the NT2 teratocarcinoma cell line suggest its role in early neuronal differentiation induced by retinoic acid. WNT3A is clustered with WNT3 on chromosome 17q21.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.