Thermo Fisher Scientific IL-31 Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific IL-31 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
SHTLPVRLLR PSDDVQKIVE ELQSLSKMLL KDVEEEKGVL VSQNYTLPCL SPDAQPPNNI HSPAIRAYLK TIRQLDNKSV IDEIIEHLDK LIFQDAPETN ISVPTDTHEC KRFILTISQQ FSECMDLALK SLTSGAQQAT T.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-31. Anti-Human IL-31-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hIL-31 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-31 (500-P249) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hIL-31.

Western Blot:
To detect hIL-31 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant hIL-31 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package Information:
500-P249BT-1MG will be provided as 2 × 500 µg.

Target Information

Interleukin-31 (IL-31) is a recently discovered T-cell cytokine closely related to IL-6 type cytokines and is preferentially produced by T helper type 2 cells. IL-31 activity is mediated through the ligand-induced oligomerization of a dimeric receptor complex containing IL-31 receptor A and oncostatin M receptor. In response to IL-31 binding, these proteins activate the JAK/STAT and AKT signaling pathways. RNA levels of IL-31 receptor A and oncostatin M receptor are induced in activated monocytes but are expressed constitutively in epithelial cells. IL-31 overexpression in transgenic mice results in pruritis, alopecia, and skin lesions; in humans, it may contribute to atopic dermatitis, suggesting IL-31 as a potential target for antipruritic drug development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지: 500-P249BT-500UG_IL-31_Q6EBC2-1_Rabbit.svg)
(이미지: 500-P249BT-500UG_IL-31_Q6EBC2-1_Rabbit_PDP.jpeg)