Thermo Fisher Scientific IL-31 Polyclonal Antibody, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.5–2.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
SHTLPVRLLR PSDDVQKIVE ELQSLSKMLL KDVEEEKGVL VSQNYTLPCL SPDAQPPNNI HSPAIRAYLK TIRQLDNKSV IDEIIEHLDK LIFQDAPETN ISVPTDTHEC KRFILTISQQ FSECMDLALK SLTSGAQQAT T

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-31. Anti-Human IL-31-specific antibody was purified by affinity chromatography employing an immobilized Human IL-31 matrix.

Sandwich ELISA:
To detect Human IL-31 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-31 (500-P249Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human IL-31.

Western Blot:
To detect Human IL-31 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-31 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Interleukin-31 (IL-31) is a T-cell cytokine closely related to IL-6 type cytokines and is preferentially produced by T helper type 2 cells. IL-31 activity is mediated through the ligand-induced oligomerization of a dimeric receptor complex containing IL-31 receptor A and oncostatin M receptor. These proteins activate the JAK/STAT and AKT signaling pathways. RNA levels of IL-31 receptor A and oncostatin M receptor are induced in activated monocytes and constitutively expressed in epithelial cells. Overexpression of IL-31 in transgenic mice causes pruritis, alopecia, and skin lesions; in humans, IL-31 may be involved in atopic dermatitis, suggesting it as a potential target for antipruritic drug development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.