Thermo Fisher Scientific IL-4 Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL
• Detection limit: 1.5–3.0 ng/lane (reducing or non-reducing conditions)

ELISA

• Tested Dilution: 0.25–1.0 µg/mL
• Sandwich ELISA: Detects 0.2–0.4 ng/well of Recombinant hIL-4 using PeproTech Polyclonal Anti-Human IL-4 (500-P24) as capture antibody

Product Specifications

Product Specific Information

AA Sequence of Recombinant Protein:
MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-4. Anti-Human IL-4-specific antibody was purified by affinity chromatography and then biotinylated.

Western Blot:
To detect hIL-4 by Western Blot, use at 0.1–0.2 µg/mL. Detection limit for Recombinant hIL-4 is 1.5–3.0 ng/lane.

Sandwich ELISA:
For hIL-4 detection, use 0.25–1.0 µg/mL antibody solution. In conjunction with PeproTech Polyclonal Anti-Human IL-4 (500-P24), detects 0.2–0.4 ng/well Recombinant hIL-4.

Package:
500-P24BT-1MG is provided as 2 × 500 µg.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells. It serves as a ligand for the interleukin 4 receptor, which also binds IL13, contributing to overlapping functions. STAT6 plays a key role in mediating IL-4 signaling. The IL-4 gene, along with IL-3, IL-5, IL-13, and CSF2, forms a cytokine gene cluster on chromosome 5q. IL-4 is located near IL13 and regulated by long-range elements over a 120 kb region. IL-4 delta 2 is a known splice variant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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