Thermo Fisher Scientific CCL5 (RANTES) Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
SPYGSDTTPC CFAYLSLALP RAHVKEYFYT SSKCSNLAVV FVTRRNRQVC ANPEKKWVQE YINYLEMS

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine RANTES (CCL5). Anti-Murine RANTES (CCL5)-specific antibody was purified by affinity chromatography employing an immobilized Murine RANTES (CCL5) matrix.

Sandwich ELISA:
To detect Murine RANTES (CCL5) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required.
This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine RANTES (CCL5) (500-P118Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Murine RANTES (CCL5).

Western Blot:
To detect Murine RANTES (CCL5) by Western Blot, use at 0.1–0.2 µg/mL.
With compatible secondary reagents, the detection limit for Recombinant Murine RANTES (CCL5) is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Target Information

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are secreted proteins involved in immunoregulatory and inflammatory processes.
CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It induces histamine release from basophils and activates eosinophils.
This cytokine is a major HIV-suppressive factor produced by CD8+ cells and acts as a natural ligand for CCR5, suppressing in vitro replication of R5 strains of HIV-1.
Alternative splicing results in multiple transcript variants encoding different isoforms.
Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.