Thermo Fisher Scientific CCL5 (RANTES) Polyclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL

View 4 publications 4 publications

In vitro Assay (IV)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 3 publications 3 publications

Immunostaining (IS)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse, Rat

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Murine RANTES (CCL5) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CCL5 (RANTES),uniProtId:P30882-1,ncbiNodeId:10090,antigenRange:24-91,antigenLength:91,antigenImageFileName:500-P118-100UG_CCL5_RANTES_P30882-1_Rabbit.svg,antigenImageFileNamePDP:500-P118-100UG_CCL5_RANTES_P30882-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929300

Product Specific Information

AA Sequence of recombinant protein: SPYGSDTTPC CFAYLSLALP RAHVKEYFYT SSKCSNLAVV FVTRRNRQVC ANPEKKWVQE YINYLEMS.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine RANTES (CCL5). Anti-Murine RANTES (CCL5)-specific antibody was purified by affinity chromatography employing an immobilized Murine RANTES (CCL5) matrix.

Sandwich ELISA: To detect Murine RANTES (CCL5) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine RANTES (CCL5) (500-P118Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine RANTES (CCL5).

Western Blot: To detect Murine RANTES (CCL5) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine RANTES (CCL5) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.