Thermo Fisher Scientific IFN gamma Polyclonal Antibody, PeproTech
마우스 IFN-γ를 특이적으로 인식하는 Rabbit Polyclonal 항체로, Western blot, ELISA, IHC 등 다양한 실험에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. 연구용으로 면역반응 및 사이토카인 조절 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 2 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Flow Cytometry (Flow) | – | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | View 5 publications |
| Neutralization (Neu) | – | View 2 publications |
| Blocking Assay (BLOCK) | – | View 1 publication |
| In vitro Assay (IV) | – | View 11 publications |
| Immunostaining (IS) | – | View 5 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Murine IFN-gamma |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929306 |
Product Specific Information
AA Sequence of recombinant protein:
MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IFN-gamma. Anti-Murine IFN-gamma-specific antibody was purified by affinity chromatography employing an immobilized Murine IFN-gamma matrix.
Sandwich ELISA:
To detect Murine IFN-gamma by sandwich ELISA (100 µL/well antibody solution), use 0.5–2.0 µg/mL of this antibody. When used with PeproTech Biotinylated Anti-Murine IFN-gamma (500-P119Bt) as a detection antibody, it allows detection of 0.2–0.4 ng/well of Recombinant Murine IFN-gamma.
Western Blot:
For Western Blot analysis, use at 0.1–0.2 µg/mL. Detection limit is 1.5–3.0 ng/lane under reducing or non-reducing conditions, when used with compatible secondary reagents.
Target Information
IFN gamma (Interferon gamma, Type II interferon)
- Macrophage activation factor and immune interferon produced by T-lymphocytes and NK cells.
- Dimeric glycoprotein (~45 kDa) consisting of two 146 amino acid subunits.
- Exhibits species-specific biological activity; human IFN gamma does not cross-react with mouse.
- Functions: antiviral and antiproliferative activity, MHC class I/II induction, macrophage activation, and enhanced B-cell immunoglobulin secretion.
- Involved in cytokine regulation and activates the JAK-STAT pathway via IFN gamma receptor I and II.
- Approximately 40% sequence homology between human and mouse IFN gamma.
- Upregulated by IL-2, FGF basic, EGF; downregulated by vitamin D3 or DMN.
- IFN gamma gene mutations are associated with aplastic anemia.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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