Thermo Fisher Scientific IGF1 Polyclonal Antibody, Biotin, PeproTech
Biotin 결합된 Rabbit Polyclonal Anti-Human IGF1 항체로 Western blot과 ELISA 분석에 적합. 고순도 재조합 IGF-I 항원으로 면역화 후 친화 크로마토그래피로 정제. 세포 성장, 분화, 생존 연구용으로 사용 가능. -20°C에서 보관.
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Thermo Fisher Scientific IGF1 Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human IGF-I |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929313 |
Product Specific Information
AA Sequence of recombinant protein:
GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IGF-I. Anti-Human IGF-I-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
For detection of Human IGF-I by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL is required. This biotinylated polyclonal antibody, used with PeproTech Polyclonal Anti-Human IGF-I (500-P11) as a capture antibody, detects at least 0.2–0.4 ng/well of Recombinant Human IGF-I.
Western Blot:
For detection of hIGF-I by Western Blot, use at a concentration of 0.1–0.2 µg/mL. Detection limit for Recombinant hIGF-I is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Packaging:
500-P11BT-1MG is provided as 2 × 500 µg.
Target Information
IGF1 (Insulin-like Growth Factor-1) is structurally and functionally related to insulin but exhibits higher growth-promoting activity. It induces various cellular responses such as proliferation, differentiation, migration, and survival. IGF1 stimulates glucose transport and glycogen/DNA synthesis in muscle, bone, and cartilage cells at lower concentrations than insulin.
In circulation, IGFs bind to IGF-binding proteins (IGFBPs) that prolong their half-life and assist in delivery to target tissues. IGF-I is a potent activator of the AKT signaling pathway, promoting proliferation and inhibiting apoptosis. Mature human IGF-I is 100% homologous with bovine and porcine proteins. Low IGF1 levels have been associated with Alzheimer’s disease. Defects in the IGF1 gene can cause insulin-like growth factor 1 deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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