Thermo Fisher Scientific IGF1 Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific IGF1 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IGF-I. Anti-Human IGF-I-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
For detection of Human IGF-I by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL is required. This biotinylated polyclonal antibody, used with PeproTech Polyclonal Anti-Human IGF-I (500-P11) as a capture antibody, detects at least 0.2–0.4 ng/well of Recombinant Human IGF-I.

Western Blot:
For detection of hIGF-I by Western Blot, use at a concentration of 0.1–0.2 µg/mL. Detection limit for Recombinant hIGF-I is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Packaging:
500-P11BT-1MG is provided as 2 × 500 µg.

Target Information

IGF1 (Insulin-like Growth Factor-1) is structurally and functionally related to insulin but exhibits higher growth-promoting activity. It induces various cellular responses such as proliferation, differentiation, migration, and survival. IGF1 stimulates glucose transport and glycogen/DNA synthesis in muscle, bone, and cartilage cells at lower concentrations than insulin.

In circulation, IGFs bind to IGF-binding proteins (IGFBPs) that prolong their half-life and assist in delivery to target tissues. IGF-I is a potent activator of the AKT signaling pathway, promoting proliferation and inhibiting apoptosis. Mature human IGF-I is 100% homologous with bovine and porcine proteins. Low IGF1 levels have been associated with Alzheimer’s disease. Defects in the IGF1 gene can cause insulin-like growth factor 1 deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.