Thermo Fisher Scientific MIP-1 gamma Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.25-1.0 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Murine MIP-1gamma (CCL9/10) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MIP-1 gamma,uniProtId:P51670-1,ncbiNodeId:10090,antigenRange:22-122,antigenLength:122,antigenImageFileName:500-P117BT-500UG_MIP-1_gamma_P51670-1_Rabbit.svg,antigenImageFileNamePDP:500-P117BT-500UG_MIP-1_gamma_P51670-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

AA Sequence of recombinant protein: QITHATETKE VQSSLKAQQG LEIEMFHMGF QDSSDCCLSY NSRIQCSRFI GYFPTSGGCT RPGIIFISKR GFQVCANPSD RRVQRCIERL EQNSQPRTYK Q.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine MIP-1gamma (CCL9/10). Anti-Murine MIP-1gamma (CCL9/10)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Murine MIP-1gamma (CCL9/10) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine MIP-1gamma (CCL9/10) (500-P117) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine MIP-1gamma (CCL9/10).

Western Blot: To detect Murine MIP-1gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine MIP-1gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P117BT-1MG will be provided as 2 x 500 µg

Target Information

MIP-1gamma is a CC chemokine found in murine blood and a wide variety of murine tissues, with no known human homolog. MIP-1gamma signals through the CCR1 receptor. MIP-1gamma chemoattracts neutrophils, and also inhibits colony formation of bone marrow myeloid immature progenitors. MIP-1gamma contains six cysteines, including the four highly conserved cysteine residues present in CC chemokines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.