Thermo Fisher Scientific IFN gamma Polyclonal Antibody, Biotin, PeproTech
마우스 IFN-γ를 인식하는 Biotin 결합 폴리클로날 항체로, ELISA 및 Western blot에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 고순도의 재조합 단백질로 면역화된 토끼에서 생산되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | Detection limit: 1.5–3.0 ng/lane (reducing or non-reducing conditions) |
| ELISA | 0.25–1.0 µg/mL | Detection limit: 0.2–0.4 ng/well (with capture antibody PeproTech 500-P119) |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Murine IFN-gamma |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IFN-gamma. The anti-Murine IFN-gamma-specific antibody was purified by affinity chromatography and subsequently biotinylated.
Sandwich ELISA:
For detection of Murine IFN-gamma, use 0.25–1.0 µg/mL of this antibody in 100 µL/well. In conjunction with PeproTech Polyclonal Anti-Murine IFN-gamma (500-P119) as the capture antibody, this combination allows detection of at least 0.2–0.4 ng/well of Recombinant Murine IFN-gamma.
Western Blot:
For Western blot analysis, use at 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Murine IFN-gamma is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Packaging:
500-P119BT-1MG is provided as 2 × 500 µg vials.
Target Information
IFN gamma (Interferon gamma, Type II interferon)
IFN gamma is a macrophage activation factor produced mainly by T-lymphocytes and natural killer cells in response to antigens, mitogens, and other cytokines. It is a dimeric glycoprotein (~45 kDa) composed of two 146 amino acid subunits. On SDS-PAGE, it appears as 25, 20, and minor 15.5 kDa bands due to differential glycosylation.
Functions and Characteristics:
- Exhibits antiviral and tumor antiproliferative activity
- Induces MHC class I and II expression
- Activates macrophages and enhances B lymphocyte immunoglobulin secretion
- Species-specific activity (human IFN gamma does not cross-react with mouse)
- Activates the JAK-STAT pathway via IFN gamma receptor I and II
- Upregulated by IL2, FGF basic, and EGF; downregulated by vitamin D3 or DMN
- Mutations in the IFN gamma gene are associated with aplastic anemia
Note: For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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