Thermo Fisher Scientific Phospho-alpha Synuclein (Ser129) Recombinant Rabbit Monoclonal Antibody (J18), FITC
Phospho-alpha Synuclein (Ser129) 검출용 FITC 결합 리컴비넌트 토끼 단클론 항체. 인간 및 생쥐 반응성, HEK293 발현 시스템 사용. WB, IHC, ICC/IF, ELISA 등 다양한 응용 가능. 형광 최대치 498/517 nm, 4°C 암소 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
| ELISA | Assay-dependent |
| Dot blot (DB) | 1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | J18 |
| Immunogen | Human alpha synuclein AA 124–134: AYEMP-pS-EEGYQ-Cys |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 9.09 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932385 |
Available Formats
- APC (Cat. MA5-45930)
- PerCP (Cat. MA5-45932)
- Custom conjugation available upon request
Product Specific Information
- A 1:500 dilution of MA5-45931 was sufficient for detection of Alpha Synuclein pSer129 in 10 µg of Mouse Brain by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
- Binds to phosphorylated serine 129 on alpha synuclein.
- Does not detect unphosphorylated serine 129 alpha synuclein.
Target Information
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. These proteins are abundant in the brain and play a role in regulating synaptic transmission and membrane trafficking. Alpha- and beta-synuclein selectively inhibit phospholipase D2, suggesting involvement in signaling pathways.
Defects in the SNCA gene, encoding alpha-synuclein, are implicated in Parkinson’s disease. Alpha-synuclein peptides are major components of amyloid plaques in Alzheimer’s disease. Multiple alternatively spliced transcripts encoding different isoforms have been identified.
Diseases associated with SNCA include Parkinson Disease 1 (Autosomal Dominant) and Dementia, Lewy Body.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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