Thermo Fisher Scientific PSEN2 Monoclonal Antibody (APS 21)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Immunocytochemistry (ICC/IF)

0.75 µg/mL

-

ELISA (ELISA)

4.5 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

APS 21

Immunogen

Synthetic peptide corresponding to residues C(31) Q E G R Q G P E D G E N T A(45) of human PS2. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PSEN2,uniProtId:P49810-1,ncbiNodeId:9606,antigenRange:32-45,antigenLength:448,antigenImageFileName:MA1-753_PSEN2_P49810-1_House_mouse.svg,antigenImageFileNamePDP:MA1-753_PSEN2_P49810-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325092

Product Specific Information

MA1-753 detects presenilin 2 protein (PS2) from rat, mouse, human, and nonhuman primate samples. No cross-reactivity is seen with presenilin 1.

MA1-753 has successfully been used in immuno-fluorescence, Western blot and ELISA procedures. By Western blot, this antibody detects an ~28 kDa protein representing PS2 NT (N-terminus fragment) and the ~46 kDa full-length PS2 from transfected COS-7 cells. In 4% paraformaldehyde fixed COS-7 cells transfected with hPS2, MA1-753 showed specific Golgi, nuclear envelope, and ER labeling.

The MA1-753 immunogen is a synthetic peptide corresponding to residues C(31) Q E G R Q G P E D G E N T A(45) of human PS2.

Target Information

Alzheimer`s disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1 or PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor such that, they either directly regulate gamma-secretase activity, or themselves act are protease enzymes. Two alternatively spliced transcript variants encoding different isoforms of PSEN2 have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.