Thermo Fisher Scientific Presenilin 1 Monoclonal Antibody (APS 18)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50-1:500

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20 - 1:200

-

Immunocytochemistry (ICC/IF)

1:50 - 1:200

View 1 publication 1 publication

ELISA (ELISA)

1:1,000

-

Immunoprecipitation (IP)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

APS 18

Immunogen

Synthetic peptide corresponding to residues C S(313) K Y N A E S T E R E S Q D T V A E N D D G(334) of humen PS1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Presenilin 1,uniProtId:P49768-1,ncbiNodeId:9606,antigenRange:313-334,antigenLength:467,antigenImageFileName:MA1-752_Presenilin_1_P49768-1_House_mouse.svg,antigenImageFileNamePDP:MA1-752_Presenilin_1_P49768-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2170605

Product Specific Information

MA1-752 detects presenilin 1 protein (PS1) from mouse, rat, human, and nonhuman primate samples. No cross-reactivity is seen with presenilin 2.

MA1-752 has successfully been used in immuno-fluorescence, immunocytochemistry, Western blot, immunoprecipitation, and ELISA procedures. By Western blot, this antibody detects an ~18 kDa protein representing PS1 CT (C-terminus fragment) and the ~46 kDa full-length PS1 from transfected SH-SY5Y cells. In 4% paraformaldehyde fixed N2a cells, MA1-752 showed endogenous PS1 staining in the Golgi and cellular vesicles.

The MA1-752 immunogen is a synthetic peptide corresponding to residues C S(313) K Y N A E S T E R E S Q D T V A E N D D G(334) of humen PS1.

Target Information

Presenilin1 was initially identified a marker of susceptibility to early-onset Alzheimers disease. In addition to PEN2, nicastrin and APH-1, Presenilin1 forms the -gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimers disease. Like the tumor necrosis factor-alpha-converting enzyme (TACE) and the beta-site cleavage enzyme (BACE) protease families, -gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and -gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and -gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer`s disease. Multiple isoforms of presenilin1 are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.