Thermo Fisher Scientific F4/80 Monoclonal Antibody (Cl:A3-1)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-dependent

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:100

View 2 publications 2 publications

Flow Cytometry (Flow)

Neat-1:10

View 5 publications 5 publications

Immunoprecipitation (IP)

Assay-dependent

-

Radioimmune Assays (RIA)

Assay-dependent

-

Immunomicroscopy (IM)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

Cl:A3-1

Immunogen

Thioglycollate stimulated peritoneal macrophages from C57/BL mice. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:F4/80,uniProtId:Q61549-1,ncbiNodeId:10090,antigenRange:1-931,antigenLength:931,antigenImageFileName:MA5-16624_F480_Q61549-1_Rat.svg,antigenImageFileNamePDP:MA5-16624_F480_Q61549-1_Rat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• APC
• Biotin
• FITC
• PE
• Request custom conjugation

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

tissue culture supernatant/0.2M tris HCl, pH 7.4, with 5-10% FBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2538120

Product Specific Information

This product requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hrs. Citrate buffer (pH 6.0) is recommended for tissues fixed for more than 24 hrs. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Rat anti mouse F4/80 antibody, clone Cl:A3-1 recognizes the murine F4/80 antigen, a approximately 160 kDa cell surface glycoprotein member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).

Target Information

F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.