Thermo Fisher Scientific c-Myc Oncoprotein Monoclonal Antibody (9E10.3)
c-Myc 단백질을 특이적으로 인식하는 Mouse monoclonal antibody로, 다양한 암 연구에 활용 가능. IHC, ICC/IF, Flow Cytometry 등 다중 응용에 적합. Human에 반응하며 Mouse, Rat, Chicken에는 비반응성. 고순도 Protein A/G 정제, 1 mg/mL 농도.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9E10.3 |
| Immunogen | Synthetic peptide corresponding to aa 408–439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-Myc, coupled to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Does not react with Mouse, Rat or Chicken.
Target Information
The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is commonly activated in various tumor cells and plays key roles in cellular proliferation, differentiation, apoptosis, and cell cycle progression.
Phosphorylation at Thr58/Ser62 by kinases such as GSK3, CDK, ERK2, and JNK is associated with regulation of cell proliferation.
c-Myc is essential for vasculogenesis and angiogenesis in tumor development. Deregulated c-Myc expression induces apoptosis and is linked to multiple cancers including lung, breast, colon, and Burkitt’s lymphoma.
Antibodies against c-Myc epitopes recognize overexpressed proteins containing Myc epitope tags fused to amino or carboxy termini of target proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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