Thermo Fisher Scientific c-Myc Oncoprotein Monoclonal Antibody (9E10.3)
c-Myc 단백질을 인식하는 마우스 단클론 항체로, IHC, ICC, Flow Cytometry 등 다양한 응용에 적합합니다. 인간 시료에 반응하며, 고순도 Protein A/G 정제 및 안정한 PBS 버퍼에 보관됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9E10.3 |
| Immunogen | A synthetic peptide corresponding to aa 408–439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-Myc, coupled to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C, do not freeze |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Does not react with Mouse, Rat or Chicken.
Target Information
The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is commonly activated in various tumor cells and plays critical roles in cell proliferation, differentiation, apoptosis, and cell cycle progression.
Phosphorylation at Thr58/Ser62 by kinases such as GSK3, CDK, ERK2, and JNK regulates c-Myc function in proliferation and cell cycle control.
c-Myc is essential for vasculogenesis and angiogenesis in tumor development. Deregulated c-Myc expression induces apoptosis in different cell types.
Antibodies against c-Myc epitopes recognize overexpressed proteins containing Myc tags fused to target proteins.
Amplification or translocation of the c-Myc gene is associated with various human cancers, including lung, breast, colon carcinomas, and Burkitt’s lymphoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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