Thermo Fisher Scientific Human Caspase 9 Synthetic Peptide

Applications

Control (Ctrl)

• Assay-dependent

Blocking Assay (BLOCK)

• Assay-dependent

Product Specifications

Product Specific Information

This peptide corresponds to 20 amino acids near the center of human Caspase-9.
PEP-0030 can be used as a blocking peptide with polyclonal antibody PA5-19904.

Target Information

Caspase 9 (ICE-like apoptotic protease 6, ICE LAP6, apoptotic protease Mch6, apoptotic protease activating factor 3, Apaf3) is a member of the peptidase family C14 that contains a CARD domain.
Caspases are cysteine proteases expressed as inactive precursors that mediate apoptosis by proteolysis of specific substrates. Caspase 9 is active as a heterotetramer, present in the cytosol, and upon activation translocates to the mitochondria.
It is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Binding of Caspase-9 to Apaf-1 leads to activation of the protease, which then cleaves and activates Caspase-3.
Caspase 9 promotes DNA damage-induced apoptosis in an ABL1/c-Abl-dependent manner and cleaves poly(ADP-ribose) polymerase (PARP). Caspase 9 is expressed in various human tissues.
An isoform of rat Caspase-9 has been identified where the C terminus of full-length Caspase-9 is replaced with an alternative peptide sequence. Mutations in Caspase 9 affect brain development; in mice, insufficient Caspase 9 results in abnormal cerebrum and neuron development.
In humans, dysfunctional Caspase 9 expression varies among tissues, and low levels may play a role in cancer development and neurodegenerative diseases such as Alzheimer’s Disease (AD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.