Thermo Fisher Scientific Human RIP2 Synthetic Peptide

Applications

Control (Ctrl)

• Assay-dependent

Blocking Assay (BLOCK)

• Assay-dependent

Product Specifications

Product Specific Information

This peptide corresponds to 20 amino acids at the amino terminus of human RICK.
PEP-0031 can be used as a blocking peptide with polyclonal antibody PA5-19905.

Target Information

RIPK2 is a serine/threonine kinase involved in cell activation, proliferation, differentiation, and apoptosis. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize, and recruit RIPK2 through CARD-CARD domains. RIPK2 contributes to tyrosine phosphorylation of ARHGEF2 via Src tyrosine kinase, leading to NF-κB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes Lys-63-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2, and BIRC3. This polyubiquitination mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces Lys-63-linked polyubiquitination of IKBKG/NEMO, resulting in activation of IKBKB/IKKB. Consequently, NF-κB is released from its inhibitors, translocates into the nucleus, and activates transcription of genes involved in immune response, growth control, and apoptosis protection. RIPK2 also promotes BCL10 phosphorylation and subsequent NF-κB activation during T-cell receptor engagement.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.