Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), Super Bright 645, eBioscience
CD45.2 단백질을 인식하는 Mouse monoclonal antibody (Clone 104)로, Super Bright 645 형광체가 결합된 제품입니다. Flow Cytometry에 최적화되어 있으며, 405 nm excitation / 645 nm emission 특성을 가집니다. 주요 마우스 균주(C57BL/6, BALB/c 등)에서 CD45.2 발현 분석에 적합합니다.
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Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), Super Bright 645, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 14 publications |
Product Specifications
| Specification | Description |
|---|---|
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 645 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 104 |
| Conjugate | Super Bright™ 645 |
| Excitation / Emission Max | 414 / 645 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2662421 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 605 / 650 / 711 / 786
- eFluor 450 / 506
- FITC
- NovaFluor Blue 510 / 555 / 585 / 610-70S / 660-120S
- PE / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 780
- Request custom conjugation available
Product Specific Information
The 104 monoclonal antibody reacts with the mouse CD45 molecule (leukocyte common antigen, LCA) in CD45.2-expressing mouse strains such as C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A, and AKR. CD45.2 is expressed by all leukocytes in these strains.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested with mouse splenocytes at ≤0.5 µg/test in 100 µL volume. Optimal titration recommended.
Super Bright 645 is a tandem dye excited at 405 nm and emits at 645 nm. Use a 660/20 bandpass filter and ensure instrument compatibility.
When using multiple Super Bright dye-conjugated antibodies, apply Super Bright Complete Staining Buffer (Product # SB-4401) to minimize polymer interactions.
Light Sensitivity: Protect vial and samples from light.
Fixation: Samples can be stored up to 3 days at 4°C in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) with minimal brightness loss.
Excitation: 405 nm
Emission: 645 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising ~10% of lymphocyte surface proteins. It regulates Src-family kinases, influencing lymphocyte development and antigen signaling. CD45.2 isoforms differ by extracellular domains and expression patterns. It also modulates integrin-mediated survival signaling and participates in apoptosis-related DNA fragmentation. CD45RA is an isoform with restricted lymphoid expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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