Thermo Fisher Scientific CD45RO Monoclonal Antibody (UCHL1), Super Bright 645, eBioscience
인간 CD45RO를 인식하는 UCHL1 클론 단일클론 항체로, Super Bright 645 형광체가 결합되어 있습니다. Flow Cytometry에 최적화되어 있으며, 405 nm 레이저로 여기되어 645 nm에서 방출됩니다. 메모리 T세포 및 단핵구 분석에 적합하며, 광민감성이 있어 암실 보관 필요.
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Thermo Fisher Scientific CD45RO Monoclonal Antibody (UCHL1), Super Bright 645, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.5 µg)/test
- View 6 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host/Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 645, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UCHL1 |
| Conjugate | Super Bright™ 645 |
| Excitation/Emission Max | 414 / 645 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2662467 |
Available Conjugate Formats
- Unconjugated
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 647
- Alexa Fluor Plus 750
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 661
- Brilliant Violet 650
- eFluor 450
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Super Bright 780
- Request custom conjugation
Product Specific Information
Description:
The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes, and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is commonly used to discriminate subsets of peripheral T cells.
Applications Reported:
This UCHL1 antibody has been reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended use: 5 µL (0.5 µg) per test (100 µL final volume, cell count 10⁵–10⁸ cells/test).
Super Bright 645 Characteristics:
- Tandem dye excited by violet laser (405 nm), emits at 645 nm
- Recommended filter: 660/20 bandpass
- Use Super Bright Complete Staining Buffer (Product # SB-4401) when using multiple Super Bright dyes
- Light sensitive – protect from light
- Fixation: store samples in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark
Excitation/Emission:
- Excitation: 405 nm
- Emission: 645 nm
- Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1):
- Shortest isoform of receptor-type protein tyrosine phosphatase CD45
- Crucial in lymphocyte development and antigen signaling
- Regulates Src-family kinases and promotes cell survival
- Involved in DNA fragmentation during apoptosis
- Expressed on macrophages, CD8+ T cells, activated T cells, and myeloma cells
- Isoform expression varies by cell type and physiological state
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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