Thermo Fisher Scientific Phospho-FOXO1 (Ser319) Polyclonal Antibody
FOXO1 단백질의 Ser319 인산화 부위를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF에 적합하며 인산화된 FOXO1A의 내인성 수준을 검출 가능. PBS/glycerol buffer에 보존, -20°C에서 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human FOXO1 (Accession Q12778), corresponding to amino acid residues around phosphorylated Ser319. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2816451 |
Product Specific Information
This antibody detects endogenous levels of FOXO1A only when phosphorylated at Serine 319.
Target Information
FOXO1 (FKHR, ForKHead Box O1) is a member of the forkhead homeotic gene family of transcription factors. It can act as a coactivator or corepressor of nuclear receptor activity mediated through the LXXLL motif in the carboxyl terminal region. Association with PAX3 has been implicated in alveolar rhabdomyosarcoma. FOXO1 is linked to anti-tumor activity, autophagy, and myogenic differentiation. It is a key target of insulin signaling regulating metabolic homeostasis under oxidative stress. FOXO1 binds to the insulin response element (IRE) and Daf-16 family binding element (DBE) to regulate gene expression involved in redox balance, osteoblast regulation, and glucose metabolism. In hepatocytes, FOXO1 promotes gluconeogenesis in cooperation with PPARGC1A by activating genes such as IGFBP1, G6PC, and PPCK1. It also acts downstream of CDK1, PKB/AKT1, and SKT4/MST1 to regulate cell death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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