Thermo Fisher Scientific Glucoamylase Polyclonal Antibody, HRP
Thermo Fisher Scientific의 Glucoamylase Polyclonal Antibody, HRP는 Rhizopus 유래 글루코아밀레이스에 특이적인 염소 IgG 항체입니다. Western blot, ELISA, IP에 사용 가능하며 HRP 결합형으로 민감한 검출이 가능합니다. 연구용으로 안정적이며 장기 보관 시 -20°C에서 유지됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,500 |
| ELISA | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 1:500–1:2,500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Fungi |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Glucoamylase [Rhizopus] |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Glucoamylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion exchange chromatography, followed by extensive dialysis against the specified buffer.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, as well as purified and partially purified Glucoamylase [Rhizopus].
Cross-reactivity against Glucoamylase from other sources is unknown.
Target Information
Rhizopus glucoamylase exists in multiple forms (Gluc 1, Gluc 2, and Gluc 3), all of which hydrolyze gelatinized starch at similar rates. Only Gluc 1 is able to adsorb raw starch.
It hydrolyzes terminal (1→4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains, releasing beta-D-glucose.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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