Thermo Fisher Scientific Glucoamylase Polyclonal Antibody, HRP

Applications

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot contents and freeze at -20°C or below.
• Avoid cycles of freezing and thawing.
• Centrifuge product if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

Glucoamylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion exchange chromatography, followed by extensive dialysis against the specified buffer.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, as well as purified and partially purified Glucoamylase [Rhizopus].
Cross-reactivity against Glucoamylase from other sources is unknown.

Target Information

Rhizopus glucoamylase exists in multiple forms (Gluc 1, Gluc 2, and Gluc 3), all of which hydrolyze gelatinized starch at similar rates. Only Gluc 1 is able to adsorb raw starch.
It hydrolyzes terminal (1→4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains, releasing beta-D-glucose.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.