Thermo Fisher Scientific CD323 (JAM3) Monoclonal Antibody (SHM33), eFluor 570, eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

1 µg/mL

-

Product Specifications

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

SHM33

Conjugate

eFluor™ 570 eFluor™ 570 eFluor™ 570

Excitation/Emission Max

556/569 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_2573604

Product Specific Information

Description: The SHM33 monoclonal antibody reacts with human CD323, which is also known as Junctional Adhesion Molecule (JAM) 3. A member of the Ig superfamily of transmembrane proteins, CD323 is expressed on endothelial and epithelial cells, smooth muscle cells, peripheral nerves, platelets, and some B cells. In addition, this receptor is expressed on VEGF and histamine-stimulated endothelial cells, as well as on activated T cells. CD323 is involved in neutrophil transmigration during inflammation, angiogenesis, cell polarity, and nerve conduction. CD323 interacts homo- and heterotypically with beta2 and beta3 integrins, JAM2, and the viral receptor CAR.

Applications Reported: This SHM33 antibody has been reported for use in microscopy, immunohistochemical staining, and immunocytochemistry.

Applications Tested: This SHM33 antibody has been tested by immunocytochemistry of methanol-fixed human cells and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.

Excitation: 555 nm; Emission: 570 nm

Target Information

JAM3 is involved in tight junctions and represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.