Thermo Fisher Scientific CD45R (B220) Monoclonal Antibody (RA3-6B2), eFluor 570, eBioscience
CD45R(B220) 항원을 인식하는 RA3-6B2 클론의 형광 표지 단일클론 항체입니다. Human 및 Mouse 시료에 반응하며, IHC, ICC/IF, Flow Cytometry 등에 사용 가능합니다. eFluor 570 형광체 결합으로 556/569 nm 파장에서 최적의 검출이 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 29 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2 µg/mL | 2 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1 µg/mL | 3 |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | 9 |
| Flow Cytometry (Flow) | - | 2 |
| Miscellaneous PubMed (Misc) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 570, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RA3-6B2 |
| Conjugate | eFluor™ 570 |
| Excitation / Emission Max | 556 / 569 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573598 |
Additional Formats Available
Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 561, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV series, Brilliant Violet series, eFluor series, FITC, Functional Grade, NovaFluor series, PE and PE tandems, PerCP series, Super Bright series, Custom conjugation available.
Product Specific Information
Description:
The RA3-6B2 monoclonal antibody reacts with the exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is primarily expressed by B cell lineage from early Pro-B to mature B cells. Some activated T cells, LAK cells, NK progenitors, and T cells of lpr/lpr mutant mice also express this antigen.
Applications Reported:
Reported for use in microscopy, immunocytochemistry, and immunohistochemistry (formalin-fixed paraffin-embedded or frozen tissue sections).
Applications Tested:
Tested by IHC of frozen mouse spleen; effective at ≤1 µg/mL. Optimal dilution should be determined experimentally.
Filter Recommendation:
For eFluor™ 570 conjugate, use filters capturing 570 nm emission (e.g., Excitation 545/25, 565LP, Emission 605/70). Standard Alexa Fluor™ 555 or TRITC filters are acceptable.
Excitation / Emission:
555 nm / 570 nm
Target Information
CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family, regulating various cellular processes such as cell growth, differentiation, and signal transduction. It is expressed in hematopoietic cells and is essential for T- and B-cell receptor signaling. CD45 glycoprotein plays a key role in lymphocyte development and antigen signaling, acting as a regulator of Src-family kinases. Multiple isoforms exist due to alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic regions. CD45 also modulates integrin-mediated signaling and apoptosis-related DNA fragmentation. CD45RA is a restricted isoform expressed in specific lymphoid subtypes. Dysfunction of CD45 is associated with Severe Combined Immunodeficiency and Hepatitis C.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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