Thermo Fisher Scientific CD1d Monoclonal Antibody (1B1), Brilliant Violet 480, eBioscience

Applications

• Flow Cytometry (Flow): 0.5 µg/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• Biotin
• Brilliant Violet 711
• eFluor 450
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685 / 700 / 755
• NovaFluor Yellow 610 / 660 / 700 / 755
• PE
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645
• Request custom conjugation

Product Specific Information

The 1B1 monoclonal antibody reacts with mouse CD1d, a 48 kDa glycoprotein structurally homologous to MHC class I molecules. CD1d associates with β2-microglobulin (β2m) and functions similarly to MHC class II. The 1B1 antibody detects CD1d on mouse leukocytes and recognizes β2m-associated CD1d.

• Reported Application: Flow cytometric analysis
• Tested Application: Flow cytometric analysis of mouse splenocytes
• Recommended Usage: ≤ 0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
• Optimization: Titrate for optimal performance

Fluorochrome Information:
Brilliant Violet™ 480 (BV480) emits at 479 nm and is suitable for cytometers equipped with a 405 nm violet laser. Ensure instrument compatibility before use.

Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies (e.g., Super Bright, Brilliant Violet™), use Super Bright Complete Staining Buffer (#SB-4401-42) or Brilliant Stain Buffer™ (#00-4409-75) to minimize non-specific interactions.

Fixation Compatibility:
Brilliant Violet™ 480 (BV480) is not compatible with methanol-based fixation.

• Excitation: 440 nm
• Emission: 479 nm
• Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD1d is a heavy chain associated with β2-microglobulin on cortical thymocytes. Independent expression has also been observed on human intestinal epithelial cells. It is the sole group 2 member of the CD1 family of MHC-like glycoproteins. CD1d proteins present lipid and glycolipid antigens to T cells and are encoded by genes on chromosome 1. CD1d localizes to late endosomes and lysosomes via a tyrosine-based motif in its cytoplasmic tail. Diseases associated with CD1D include Mycobacterium malmoense infection and autoimmune disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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