Thermo Fisher Scientific CD79a Monoclonal Antibody (24C2.5), eBioscience

Thermo Fisher Scientific CD79a Monoclonal Antibody (24C2.5), eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• eFluor 660
• FITC
• Request custom conjugation

Product Specific Information

This 24C2.5 monoclonal antibody reacts with the intracellular tail of mouse CD79a (also known as Ig alpha or mb-1), a signaling component of the B cell receptor (BCR).
CD79a heterodimerizes with CD79b (Ig beta, B29), and together with surface Ig, make up the BCR. Both CD79 subunits consist of a single extracellular Ig domain, a transmembrane domain, and an intracellular signaling domain.
CD79a is expressed almost exclusively on B cells, as well as in most B lineage acute lymphoblastic leukemias.
The cytoplasmic domain of CD79a contains immunoreceptor tyrosine-based activation motifs (ITAMs), which constitute the signal transducing portions of the BCR. Tyrosine phosphorylation of these ITAMs by Syk and Lyn initiates numerous signaling cascades, resulting in B cell activation, proliferation, and differentiation.

Applications Reported: Used in immunoblotting (WB).
Applications Tested: Tested by western blot of reduced mouse spleen lysates at 1–10 µg/mL. Antibody should be titrated for optimal performance.
Purity: >90%, determined by SDS-PAGE.
Aggregation: <10%, determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD79a is a disulphide-linked heterodimer that includes B29 (CD79b) polypeptide. It is a B lymphocyte antigen receptor with an antigen-specific surface component Ig (immunoglobulin) that associates with Ig-alpha and Ig-beta, necessary for expression and function of the B-cell antigen receptor.
CD79a first appears at the pre-B cell stage and persists until the plasma cell stage, where it is found as an intracellular component.
It is expressed in most precursor B cell leukemias, B cell lines, B cell lymphomas, and some myelomas, but absent in myeloid or T cell lines.
Diseases associated with CD79a include Agammaglobulinemia 3 and Agammaglobulinemia, Non-Bruton type.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.