Thermo Fisher Scientific CD54 Polyclonal Antibody
Rabbit polyclonal antibody against human CD54 (ICAM-1). Western blot validated. Recognizes recombinant protein (aa 253–367). Suitable for detecting ICAM-1 expression in various human cell types. For research use only.
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Applications
- Western Blot (WB): 0.1–1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encoding aa 253–367 of human CD54 (ICAM-1, UniProt ID: P05362-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Tonsil
- Cellular location: Cell membrane
Target Information
ICAM-1 (CD54) is an 85–110 kDa single-chain type I integral membrane glycoprotein with an extracellular domain of five immunoglobulin-like repeats, a transmembrane region, and a cytoplasmic domain. It has seven potential N-linked glycosylation sites and shares homology with ICAM-2 (CD102) and ICAM-3 (CD50).
ICAM-1 binds to integrins CD11a/CD18 (LFA-1) and CD11b/CD18 (Mac-1), serving as a receptor for Rhinovirus. It is expressed by activated endothelial cells, epithelial cells, fibroblasts, chondrocytes, B and T lymphocytes, monocytes, macrophages, dendritic cells, and neutrophils. Expression increases during inflammation and is also found in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in plasma and elevated in inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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