Thermo Fisher Scientific IL-13 Monoclonal Antibody (eBio13A), APC-eFluor 780, eBioscience
마우스 IL-13을 인식하는 단클론 항체로, APC-eFluor 780 형광 표지. Flow cytometry에 최적화되어 있으며, IHC 및 ELISA에도 사용 가능. 0.2 mg/mL 농도의 액상 형태로 제공되며, 4°C 암소 보관 권장. 연구용으로만 사용.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-13 Monoclonal Antibody (eBio13A), APC-eFluor 780, eBioscience
Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.125 µg/test | View 19 publications |
| ELISA | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), APC-eFluor™ 780, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio13A |
| Conjugate | APC-eFluor™ 780 |
| Excitation/Emission Max | 756 / 785 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2716963 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- Brilliant UV 737
- Brilliant UV 805
- eFluor 450
- eFluor 660
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
Description:
The eBio13A antibody reacts with mouse IL-13. IL-13 is a cytokine produced mainly by Th2 cells and antigen-primed CD8 T cells. It is involved in allergic inflammation, parasitic clearing, and has context-dependent effects in cancer models. In humans, IL-13 regulates isotype switching in B cells and down-modulates macrophage activity by reducing pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12).
Applications Reported:
Used for intracellular staining followed by flow cytometric analysis.
Applications Tested:
Tested by intracellular staining and flow cytometry of Th2-polarized mouse splenocytes. Recommended ≤0.125 µg/test (100 µL final volume). Optimal titration recommended per assay.
Fluorochrome Information:
APC-eFluor™ 780 emits at 780 nm, excited by Red laser (633 nm). Ensure instrument compatibility.
Light Sensitivity:
Sensitive to photo-induced oxidation. Protect from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark. Clone-specific performance should be determined empirically.
Excitation / Emission / Laser:
633–647 nm / 780 nm / Red Laser.
Target Information
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. It regulates inflammatory and immune responses by down-regulating macrophage activity and inhibiting pro-inflammatory cytokine production. IL-13 is implicated in allergen-induced asthma, B cell maturation, and IgE isotype switching. Structurally, IL-13 resembles IL-3, IL-4, IL-5, and GM-CSF. The IL-13 gene is located near IL-4 on chromosome 5q within the cytokine gene cluster.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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