Thermo Fisher Scientific DLAT Monoclonal Antibody (4G9)
DLAT 단백질을 인식하는 Mouse monoclonal antibody (clone 4G9). Western blot, ICC/IF, IP에 적합하며, Human 및 Mouse 시료에 반응. 항원 친화 크로마토그래피로 정제된 고순도 항체로 연구용에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:300 |
| Immunoprecipitation (IP) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4G9 |
| Immunogen | Recombinant full length Human DLAT |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2717301 |
Product Specific Information
The antibody was affinity-purified from mouse ascites by affinity chromatography using an epitope-specific immunogen. The purity is >95% as determined by SDS-PAGE.
Target Information
DLAT encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC), located in the inner mitochondrial membrane. PDC catalyzes the conversion of pyruvate to acetyl coenzyme A. The DLAT protein, dihydrolipoamide acetyltransferase, transfers acetyl groups to coenzyme A. This protein is the antigen for antimitochondrial antibodies, which are present in nearly 95% of patients with primary biliary cirrhosis (PBC), an autoimmune liver disease that can progress to cirrhosis and liver failure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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