Thermo Fisher Scientific DGCR8 Polyclonal Antibody
DGCR8 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, WB, IHC, ICC, IP 등 다양한 응용에 적합합니다. 인간, 마우스, 랫트 반응성 보유하며 고순도(>95%)로 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | View 1 publication |
| Immunoprecipitation (IP) | 1:20–1:50 | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein of human DGCR8 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2717298 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select optimal candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the large rabbit immune repertoire
Purity is >95% by SDS-PAGE.
Target Information
The DGCR8 gene encodes a subunit of the microprocessor complex involved in microRNA biogenesis from primary transcripts. The encoded protein is a double-stranded RNA-binding protein functioning as the non-catalytic subunit of the complex. It binds double-stranded RNA substrates and facilitates cleavage by the ribonuclease III protein Drosha.
Alternate splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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