Thermo Fisher Scientific Human IgG4 Recombinant Rabbit Monoclonal Antibody (4H3)
Human, Mouse, Rat에 반응하는 재조합 토끼 단클론 항체로, Human IgG4를 인식합니다. Western blot 및 IHC에 사용 가능하며, 높은 특이성과 로트 간 일관성을 제공합니다. 항원 친화 크로마토그래피로 정제되었으며, 연구용으로 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4H3 |
| Immunogen | Synthetic peptide corresponding to Human IgG4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2717300 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select optimal clones for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the large rabbit immune repertoire
Purity: >95% by SDS-PAGE.
Target Information
Human IgG4 (human immunoglobulin G4) is a subclass of human IgG antibodies produced in response to antigens. It is the least abundant IgG subclass in blood and plays a minor role in pathogen defense.
IgG4 binds to specific antigens and promotes their clearance through mechanisms such as phagocytosis and complement activation. It is also involved in immune tolerance and inflammation suppression.
Human IgG4 is used in research to study immune responses, and to develop diagnostic and therapeutic approaches for infectious and autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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