Thermo Fisher Scientific ABCG1 Recombinant Rabbit Monoclonal Antibody
ABCG1 단백질을 인식하는 재조합 토끼 단일클론 항체로, WB, IHC, ICC/IF 등에 적합합니다. 높은 특이성과 재현성을 제공하며, HEK293 발현 시스템으로 생산되었습니다. 인간, 마우스, 랫트 시료에 반응하며, 연구용으로 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein of human ABCG1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography, Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2717295 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Purity is >95% by SDS-PAGE.
Target Information
ATP-binding cassette (ABC) transporter genes regulate the amount of dietary cholesterol retained in the body. The ABC transporter ABCG1 (ABC8) is expressed on the cell surface and in intracellular compartments of cholesterol-laden macrophages. It is induced in monocyte-derived macrophages during cholesterol influx mediated by acetylated low-density lipoprotein. In contrast, lipid efflux in cholesterol-laden macrophages mediated by HDL(3) suppresses ABCG1 expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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