Thermo Fisher Scientific Macrophage L1 Protein Monoclonal Antibody (SPM281)
Macrophage L1 단백질(Calprotectin)을 인식하는 SPM281 클론의 마우스 단클론 항체로, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합. 다종 반응성, 고순도 Protein A/G 정제, PBS 기반 액상 형태로 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Horse, Cat, Guinea pig, Goat, Human, Mouse, Non-human primate, Pig, Rabbit, Rat |
| Host/Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM281 |
| Immunogen | Affinity purified monocyte membrane preparation (target family: Calprotectin, UniProt IDs: P06702-1, P05109-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping conditions | Wet ice |
Target Information
The S100A8/A9 heterocomplex, also known as MRP-8/MRP-14 complex or calprotectin, constitutes about 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages, and ileal tissue eosinophils.
Peripheral blood monocytes carry the antigen both extra- and intracellularly, while neutrophils carry it only intracellularly.
The S100A8/A9 complex exhibits antibacterial, antifungal, immunomodulating, and antiproliferative effects, and acts as a potent chemotactic factor for neutrophils.
Elevated plasma concentrations are associated with diseases involving increased neutrophil activity, such as inflammatory bowel disease.
Calprotectin can also be detected in feces, and elevated levels have been observed in plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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