Thermo Fisher Scientific Calprotectin/MRP14/S100A9/Calgranulin B Monoclonal Antibody (MAC387)
Calprotectin/MRP14/S100A9/Calgranulin B 단백질을 인식하는 MAC387 클론의 마우스 단클론 항체. 다양한 종에서 반응하며 IHC, ICC, Flow Cytometry에 사용 가능. 단백질 A/G 정제, PBS 기반 용액 형태로 제공. 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Horse, Cat, Guinea pig, Goat, Human, Mouse, Non-human primate, Pig, Rabbit, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MAC387 |
| Immunogen | Affinity purified monocyte membrane preparation (Calprotectin: UniProt P06702-1, P05109-1; antigen range 1–114, 1–93) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4 °C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The S100A8/A9 heterocomplex, also known as MRP-8/MRP-14 complex or calprotectin, constitutes about 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages, and ileal tissue eosinophils.
Peripheral blood monocytes carry the antigen both extra- and intracellularly, while neutrophils carry it only intracellularly.
The S100A8/A9 complex exhibits antibacterial, antifungal, immunomodulating, and antiproliferative effects, and serves as a potent chemotactic factor for neutrophils.
Elevated plasma concentrations are associated with increased neutrophil activity, such as in inflammatory bowel disease.
Calprotectin is detectable in feces and other body fluids (plasma, saliva, gingival crevicular fluid, stools, synovial fluid) during infection and inflammatory conditions.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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