Thermo Fisher Scientific Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Biotin
Thermo Fisher Scientific의 Donkey anti-Goat IgG (H+L) Biotin 항체는 염소 IgG 검출용 크로스 흡착된 폴리클로날 2차 항체입니다. Western blot, ICC/IF, Flow cytometry, ELISA에 적합하며, 높은 특이성과 신호 증폭 효과를 제공합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Goat |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins, Heavy and Light chains |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term); -20°C (long term), avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534683 |
| Target | IgG |
| Cross Adsorption | Against human, mouse, rabbit, and rat IgG |
| Antibody Form | Whole Antibody |
Target Information
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins. They are useful for detection, sorting, or purification of goat IgG targets.
Secondary antibodies provide versatility for various detection systems (HRP, AP, fluorescence) and enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, these antibodies are produced by immunizing host animals with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Conjugate Formats
- Alexa Fluor 350
- Alexa Fluor 488
- Alexa Fluor 546
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 633
- Alexa Fluor 647
- Alexa Fluor 680
- AP
- DSB-X Biotin
- DyLight 350
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 650
- DyLight 680
- DyLight 755
- DyLight 800
- FITC
- HRP
- TRITC
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