Thermo Fisher Scientific Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve.
• Centrifuge to remove particulates.
• Prepare fresh working dilution daily.
• Store lyophilized material at 2–8°C.
• For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
• Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin goat serum immunoglobulins or IgG from human, mouse, rabbit, or rat.

Target Information

Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins. They are useful in detection, sorting, or purification of their targets.
Secondary antibodies enhance assay versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species, followed by purification and modification (e.g., immunoaffinity chromatography, fragmentation, or conjugation).

Available Conjugate Formats

• Alexa Fluor 350, 488, 546, 555, 568, 594, 633, 647, 680
• AP, Biotin, DSB-X Biotin
• DyLight 350, 488, 550, 594, 650, 680, 755, 800
• FITC, TRITC
• Custom conjugation available upon request

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.