Thermo Fisher Scientific Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
Thermo Fisher Scientific의 Donkey anti-Goat IgG (H+L) HRP 결합 2차 항체로 Western blot, IHC, ELISA에 적합합니다. 교차흡착 처리로 비특이적 반응을 최소화했으며, 고감도 신호 검출이 가능합니다. 친화 크로마토그래피로 정제된 고순도 항체입니다.
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Applications and Tested Dilutions
| Application | Dilution Range |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Goat |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2534679 |
| Target | IgG |
| Cross Adsorption | Against human, mouse, rabbit and rat IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
- Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve.
- Centrifuge to remove particulates.
- Prepare fresh working dilution daily.
- Store lyophilized material at 2–8°C.
- For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
- Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin goat serum immunoglobulins or IgG from human, mouse, rabbit, or rat.
Target Information
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins. They are useful in detection, sorting, or purification of their targets.
Secondary antibodies enhance assay versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species, followed by purification and modification (e.g., immunoaffinity chromatography, fragmentation, or conjugation).
Available Conjugate Formats
- Alexa Fluor 350, 488, 546, 555, 568, 594, 633, 647, 680
- AP, Biotin, DSB-X Biotin
- DyLight 350, 488, 550, 594, 650, 680, 755, 800
- FITC, TRITC
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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