Thermo Fisher Scientific Donkey anti-Mouse IgG (H+L) Secondary Antibody, FITC
Thermo Fisher Scientific의 Donkey anti-Mouse IgG (H+L) FITC 결합 2차 항체는 마우스 IgG 검출용으로 설계된 고감도 항체입니다. ICC/IF 및 유세포분석에 적합하며, 498/517 nm 형광 특성을 가집니다. 친화 크로마토그래피로 정제되어 높은 특이성과 안정성을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.8, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534686 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Alexa Fluor 488
- Alexa Fluor 594
- AP
- Biotin
- HRP
- Qdot 525
- Qdot 605
- Qdot 625
- Texas Red
- TRITC
- Custom conjugation available upon request
Product Specific Information
The sensitivity of each lot of antibody is confirmed using ELISA. The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP).
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful in detection, sorting, or purification of target proteins.
Secondary antibodies enhance versatility and sensitivity by allowing multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These antibodies are typically generated by immunizing the host with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to achieve high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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