Thermo Fisher Scientific Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, AP
Donkey 유래 anti-Goat IgG (H+L) 알칼라인 포스파타아제(AP) 결합 2차 항체로, Western blot, IHC, ELISA 등에 사용 가능. 사람, 마우스, 토끼, 랫트 IgG에 대한 교차 반응 최소화. 고순도 친화 크로마토그래피 정제. 연구용 전용.
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Applications and Tested Dilution
| Application | Dilution Range |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Goat |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | Alkaline Phosphatase (AP) |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 30 mM triethanolamine, 1% BSA, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term, store at -20°C (avoid freeze/thaw cycles). |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534682 |
| Target | IgG |
| Cross Adsorption | Against human, mouse, rabbit, and rat IgG |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Alexa Fluor 350, 488, 546, 555, 568, 594, 633, 647, 680
- Biotin, DSB-X Biotin
- DyLight 350, 488, 550, 594, 650, 680, 755, 800
- FITC, HRP, TRITC
- Custom conjugation available upon request
Target Information
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins. They are useful for detection, sorting, or purification of target proteins. Secondary antibodies provide versatility for various detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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