Takara Capturem Streptavidin

Capturem Streptavidin mini columns and 96-well plates take advantage of immobilized streptavidin on high-capacity Capturem membranes to enable rapid, high-quality purification or enrichment of biotinylated targets. Alternatively, biotinylated ligands bound to Capturem Streptavidin membranes can be used to pull down target molecules or complexes.

Capturem Streptavidin mini columns and 96-well plates take advantage of immobilized streptavidin on high-capacity Capturem membranes to enable rapid, high-quality purification or enrichment of biotinylated targets. Alternatively, biotinylated ligands bound to Capturem Streptavidin membranes can be used to pull down target molecules or complexes.

Streptavidin is a cell wall protein from the bacterium Streptomyces avidinii with a high affinity for biotin, and this interaction has found extensive use in the life sciences for enrichment and other applications. Capturem kits use a novel high-capacity membrane-based affinity technology that allows the workflows to be completed in just minutes at room temperature, from loading of samples to elution.

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Overview

• Simple, ultra-fast workflow: 5–15-minute room-temperature protocols
• High concentration: extremely small elution volumes yield highly concentrated complexes
• High quality: short residence times reduce the possibility of aggregation, complex dissociation, or loss of activity
• High reproducibility: consistent results from well to well
• Single use: novel membrane technology assembled into disposable spin columns reduces the risk of contamination and carryover

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Applications

• Purification or enrichment of biotinylated proteins
• Pull-down of binding partners (e.g., proteins or oligos) for a biotin-labeled ligand

Loading capacities and times for available Capturem Streptavidin formats

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.