Takara DNase I (RNase-free)

Takara DNase I (RNase-free)

Applications

• Template DNA digestion following in vitro transcription
• Genomic DNA digestion prior to RT-PCR
• Nick translation with DNA polymerase
• Shotgun DNA library construction
• Footprinting analysis

Components

Supplied Buffer (10X)

Source

Recombinant non-animal host containing bovine pancreas DNase I-encoding plasmid

Storage

–20°C

Unit Definition

One unit is defined as the amount of enzyme required to increase 260 nm absorbance by 0.001 per minute at 25°C (pH 5.0) using calf thymus DNA as the substrate (Kunitz unit).

Concentration

5 U/µl

Properties

Recombinant DNase I (RNase-free) is heat inactivated by incubating at 80°C for 10 minutes.

References

• Anderson, S. Nucleic Acids Res. 9, 3015–27 (1981).
• Galas, D. J. & Schmitz, A. Nucleic Acids Res. 5, 3157–70 (1978).
• Green, M. R., Maniatis, T. & Melton, D. A. Cell 32, 681–94 (1983).
• Kunitz, M. J. Gen. Physiol. 33, 349–62 (1950).
• Rigby, P. W., Dieckmann, M., Rhodes, C. & Berg, P. J. Mol. Biol. 113, 237–51 (1977).

Additional Product Information

Please refer to the product’s Certificate of Analysis for details on storage conditions, product components, and technical specifications.
Kit Components Lists and Certificates of Analysis are available under the Documents tab.

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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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