Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), NovaFluor Red 700, eBioscience

Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), NovaFluor Red 700, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.8 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 737
• Brilliant Violet 650
• eFluor 450
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 700
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The eBio124-1D1 monoclonal antibody reacts with human CD7 (gp40, Leu9), a 40 kDa receptor and member of the immunoglobulin gene superfamily.
CD7 is expressed on most immature and mature T lymphocytes, T cell leukemias, NK cells, and a subset of B cells. Cross-linking of CD7 modulates T cell and NK cell activity, affecting calcium flux, adhesion molecule expression, cytokine secretion, and proliferation. It directly associates with phosphoinositol 3’-kinase and induces D-3 phosphoinositide production and tyrosine phosphorylation.

A clonogenic subpopulation of CD34(+) CD38(-) cord blood cells expressing CD45RA, HLA-DR, and high CD7 levels can generate lymphoid cells (B, NK, dendritic) but not myeloid or erythroid lineages, distinguishing primitive lymphoid progenitors from pluripotent stem cells.

CD7 may cooperate with CD28 in Treg function; deficiency in both reduces Treg numbers and suppressive activity.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for use at 5 µL (0.8 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important:
NovaFluor dyes are incompatible with DNA intercalating viability dyes (PI, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be used for optimal staining. Add 5 µL per sample before combining with cells. For single-color controls, use 5 µL per 100 µL of cell sample.

NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered for optimized fluorescent signatures minimizing spillover and spread.

Excitation: 639 nm
Emission: 700 nm
Laser: 633–640 nm (Red)

Target Information

CD7 (gp40, Leu9) is a 40 kDa member of the immunoglobulin gene superfamily.
It is expressed on immature and mature T lymphocytes, T cell leukemias, NK cells, and subsets of normal and malignant B cells.
Cross-linking CD7 modulates T and NK cell activity through calcium flux, adhesion molecule expression, cytokine secretion, and proliferation.
CD7 is an important marker in leukemia diagnostics.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

제품 이미지