Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), NovaFluor Blue 690, eBioscience

Applications

• Reported Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.8 µg)/test

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Product Specifications

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Additional Formats Available

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 737
• Brilliant Violet 650
• eFluor 450
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 700
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

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Product Specific Information

The eBio124-1D1 monoclonal antibody reacts with human CD7 (gp40, Leu9), a 40 kDa receptor and member of the immunoglobulin gene superfamily.
CD7 is expressed on most immature and mature T lymphocytes, T cell leukemias, NK cells, and a subset of B cells. Cross-linking CD7 modulates T cell and NK cell activity by influencing calcium flux, adhesion molecule expression, cytokine secretion, and proliferation. CD7 directly associates with phosphoinositol 3′-kinase, and its ligation induces D-3 phosphoinositide production and tyrosine phosphorylation.

A clonogenic subpopulation of human CD34(+) CD38(-) cord blood cells expressing CD7 shows lymphopoietic potential, generating B cells, NK cells, and dendritic cells but not myeloid or erythroid cells. CD7(+) phenotype marks primitive lymphoid progenitors distinct from pluripotent stem cells.
CD7 also cooperates with CD28 in Treg function; deficiency in both reduces Treg numbers and suppressive activity.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for use at 5 µL (0.25 µg)/test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability testing.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining with NovaFluor conjugates.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, employing nucleic acid dye structures for engineered fluorescence signatures that minimize spectral spillover.

Excitation: 494 nm
Emission: 585 nm
Laser: 488 nm (Blue)

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Target Information

CD7 (gp40, Leu9) is a 40 kDa immunoglobulin superfamily member.

• N-terminal (aa1–107): homologous to Ig kappa light chains
• C-terminal: proline-rich stalk projecting Ig domain
• Expressed on immature/mature T cells, T cell leukemia, NK cells, and some B cells
• Cross-linking modulates T/NK cell activation, adhesion, cytokine secretion, and proliferation
• Associates with PI3K; ligation induces D-3 phosphoinositides and tyrosine phosphorylation
• Expression of CD7 serves as a diagnostic marker in leukemia research.

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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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