Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), NovaFluor Blue 660-120S, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.8 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 737
• Brilliant Violet 650
• eFluor 450
• FITC
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 700
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Custom conjugation available

Product Specific Information

Description:
The eBio124-1D1 monoclonal antibody reacts with human CD7 (gp40, Leu9), a 40 kDa receptor and member of the immunoglobulin gene superfamily. The N-terminal domain (aa1–107) is homologous to Ig kappa light chains, while the C-terminal region is proline-rich and forms a stalk projecting the Ig domain.

CD7 is expressed on most immature and mature T lymphocytes, T cell leukemias, NK cells, and a subset of B cells. Cross-linking of CD7 modulates T cell and NK cell activity, influencing calcium flux, adhesion molecule expression, cytokine secretion, and proliferation. CD7 associates with phosphoinositol 3′-kinase, inducing D-3 phosphoinositide production and tyrosine phosphorylation.

A subpopulation of CD34(+) CD38(-) cord blood cells expressing high CD7 levels shows lymphoid differentiation potential, generating B cells, NK cells, and dendritic cells but not myeloid or erythroid cells. CD7 thus helps distinguish primitive lymphoid progenitors from pluripotent stem cells.

CD7 may also cooperate with CD28 in Treg function, as double-deficient mice (CD28⁻/⁻ CD7⁻/⁻) show reduced Treg numbers and suppressive activity.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for use at 5 µL (0.8 µg) per 100 µL test sample (10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls: 5 µL per 100 µL cell sample.

NovaFluor Technology:
Based on Phiton™ technology utilizing novel nucleic acid dye structures, engineered for optimized spectral performance with minimized spillover and spread.
Excitation: 509 nm
Emission: 665 nm
Laser: 488 nm (Blue)

Target Information

CD7 (gp40, Leu9):
A 40 kDa immunoglobulin superfamily member expressed on most immature and mature T lymphocytes, T cell leukemias, NK cells, and subsets of B cells. Cross-linking CD7 modulates immune cell activation and signaling via PI3-kinase pathways. CD7 expression serves as a key marker in leukemia diagnostics.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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