Thermo Fisher Scientific VEGF-165 Monoclonal Antibody, PeproTech

Applications

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.

Preparation:
Produced in BALB/c mice immunized with highly pure Recombinant Human VEGF 165. Anti-Human VEGF 165-specific antibody was purified from cell culture by Protein A affinity chromatography.

Sandwich ELISA:
Assuming 100 µL/well, a concentration of 2.0–4.0 µg/mL of this antibody will detect at least 100 pg/mL of Recombinant Human VEGF 165 when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human VEGF 165 (500-P10BT) as the detection antibody at a concentration of approximately 0.25–1.0 µg/mL.

Western Blot:
To detect Human VEGF 165 by Western Blot analysis this antibody can be used at a concentration of 0.25–0.50 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human VEGF 165 is 0.25–0.50 ng/lane under non-reducing conditions.

Target Information

The vascular endothelial growth factor (VEGF) family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system are fundamental regulators of angiogenesis signaling. Most tumors require angiogenesis, and VEGF is a potent angiogenic cytokine associated with this process.
Five isoforms of VEGF are generated through alternative splicing from a single VEGF gene. These isoforms bind to two tyrosine-kinase receptors, flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which are primarily expressed on endothelial cells.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.