Thermo Fisher Scientific VEGF-165 Monoclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.25-0.50 µg/mL

View 2 publications 2 publications

ELISA (ELISA)

2.0-4.0 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Rat

Host/Isotype

Mouse

Class

Monoclonal

Type

Antibody

Immunogen

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View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_3074171

Product Specific Information

AA Sequence of recombinant protein: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.

Preparation: Produced in BALB/c mice immunized with highly pure Recombinant Human VEGF 165. Anti-Human VEGF 165-specific antibody was purified from cell culture by Protein A affinity chromatography.

Sandwich ELISA: Assuming 100 µL/well, a concentration of 2.0-4.0 µg/mL of this antibody will detect at least 100 pg/mL of Recombinant Human VEGF 165 when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human VEGF 165 (500-P10BT) as the detection antibody at a concentration of approximately 0.25- 1.0 µg/mL.

Western Blot: To detect Human VEGF 165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human VEGF 165 is 0.25-0.50 ng/lane, under non-reducing conditions.

Target Information

The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.