Thermo Fisher Scientific HSP70/HSC70 Monoclonal Antibody (BB70), FITC

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:2,000

-

Immunocytochemistry (ICC/IF)

1:200

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Fruit fly, Fish, Guinea pig, Hamster, Human, Mammal, Mouse, Sheep, Pig, Rabbit, Rat, Xenopus, Yeast

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

BB70

Immunogen

Chicken HSP70/HSP90 complex

Conjugate

FITC FITC FITC

View additional formats

• APC
• PerCP
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

9.1mM sodium bicarbonate/PBS, pH 7.2, with 640.91mM DMSO, 136.36mM ethanolamine

Contains

no preservative

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931554

Product Specific Information

1 µg/mL of MA5-45100 was sufficient for detection of HSP70 and HSC70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 72 (HSP) and approximately 73kDa (HSC).

Target Information

HSPA4 (Heat Shock Protein Family A (Hsp70) Member 4) is a Protein Coding gene. Diseases associated with HSPA4 include Chagas Disease and Babesiosis. Among its related pathways are Regulation of activated PAK-2p34 by proteasome mediated degradation and Cellular responses to stimuli. An important paralog of this gene is HSPH1. HSPA8 (Heat Shock Protein Family A (Hsp70) Member 8) is a Protein Coding gene. Diseases associated with HSPA8 include Auditory System Disease and Borna Disease. Among its related pathways are Processing of Capped Intron-Containing Pre-mRNA and Selective autophagy. Gene Ontology (GO) annotations related to this gene include RNA binding and ubiquitin protein ligase binding. An important paralog of this gene is HSPA2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.