Thermo Fisher Scientific HSP90 Monoclonal Antibody (8D3), APC

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:100

-

Immunoprecipitation (IP)

1:1,000

-

Product Specifications

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

8D3

Immunogen

Ah receptor (Aryl hydrocarbon receptor)

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Goat anti-mouse IgM was used to bind 25 µl of protein G-Sepharose. MA5-45106 IgM from 0.5 ml of high speed supernatant medium was loaded onto the IgG resin and incubated with 100 µl of rabbit reticulocyte lysate for 30 min. at 30C. After washing (4X1 ml), bound proteins were resolved on SDS PAGE, including HSP90, HSP70 and Hop. |Detects 90kDa. Co-immunoprecipitates HSP90 complexes, including HSP70, Hop, Ah receptors, glucocorticoid receptors, heme-regulated eukaryotic initiation factor 2 alpha (eIF-2 alpha ) kinase (HRI).

Target Information

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 and mitochondria (Chen et al., 2005 ).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.